Help! neuronal cell culture problem
serotone at replies_to_newsgroup.com
Thu Aug 3 13:50:35 EST 2000
"Don Cates" <cates at cc.umanitoba.ca> wrote in message
news:39881d93.49487258 at news.cc.umanitoba.ca...
> I've been doing cell culture of dissociated neurons from fetal rat
> medulla for several years. Recently the wheels have fallen off.
> I use 35 mm plastic dishes with a collagen (Vitrogen) coating. I then
> plate with neuron free medullary background cells for a week or more,
> then plate with my dissociated cells (mince with scissors, treat with
> trypsin for 15-20 min, gently triturate). The last few times I have done
> this, the cells seem to lift off most of the dish and form long, thick
> 'ropes' of cells which include the collagen and background cells. I've
> replaced my various media and components with fresh material with no
> luck. It is not invariable, I get the odd good dish.
> Can anyone suggest anything?
Do these ropes consist of lysed cells or healthy ones? Trypsin activity
remaining in the cells suspension would cause this.....
serotonin1 at hushmail dot com
More information about the Neur-sci