Labelling a neurotransmitter

Terry Morris tjm5+ at pitt.edu
Mon Feb 7 16:55:19 EST 2000


Please, can anyone advise or suggest alternative approaches.

I am investigating the effects of general anesthetics on the fast transport
of neurotransmitter containing vesicles in axons and the pre-synapse. Is
there a way to fluorescently label either the neurotransmitter (glutamate)
or some component of vesicular membrane so that active transport can be
recorded in real-time using confocal microscopy?

Alternatively, is it possible to label the ATP turnover of the molecular
motor (kinesin) driving vesicle movement in vivo?

Terry Morris






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