Hi folks!
Gotta grow embryonic neurons on coverslips for fluorescence microscopy
experiments with fura-2 or fluo3.
Problems:
1) Neurons don't grow very well on (poly-l-ornithine coated) GLASS
coverslips.
2) Some (all???) PLASTIC coverslips show autofluorescence which can
confuse the fluorescence measurements.
Would be very glad about any suggestions, hints etc.
Regards, Martin