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Your recommendations on PKA activators and inhibitors

Gary G. Wilson ggw5 at columbia.edu
Sat Jul 1 20:59:46 EST 2000

Try 1mM 8-Bromo-cAMP or dibuteryl-cAMP (more potent).  You can add them
via the bath as they will easily permeate the membrane.  PKA activity
should increase within a few minutes.  Walsh peptide is the standard PKA 
inhibitor.  This can be added via the pipet.  Look at a paper by Pusch
and Neher for estimating the timecourse of diffusion into the cell.  I
can dig out the reference if you need it.

On Sat, 1 Jul 2000, Zakaria
Mtchedlishvili wrote:

> Hi everybody
> I am trying to augment cAMP dependent protein kinase (PKA) activity in a
> neuron, in order to reveal it's impact on certain ionotrophic events in
> an acutely isolated dentate granule cell, during voltage clamp
> recording. For this purpose I think to include PKA catalytic subunit
> into my recording pipette. however, I don't know what should be right
> (closed to physiologically relevant) concentration of the protein. media
> etc. Also, I need to inhibit PKA. Which inhibitors and what
> concentrations are recommended?
> I will highly appreciate if someone will share his/her experience and
> suggestions on this matter.      

Gary G Wilson
Center for Molecular Recognition
Columbia University

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