nuclear extracts

Serotone serotone at replies_to_newsgroup.com
Sun Jun 11 11:56:24 EST 2000


""Jason R. Richardson"" <jrr1 at bully.net> wrote in message
news:000701bfcf2f$2d2b7ae0$78384ec6 at oemcomputer...
Does anyone know a good method for preparing nuclear extracts from rat
brain. I will be using western blotting to look at CREB and c-fos.

Sonicating the cells in a buffer containing protease inhibitors, then
centrifuging out the nuclei and cell membranes usually works.  Any other
method that produces enough shearing will probably do ok, maybe repeatedly
aspirating through a real small gauge needle would work for small amounts if
you don't have a sonicator.

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