Question for paraformaldehyde in PBS buffer

Andrew Huang d8832005 at ccunix.ccu.edu.tw
Wed Apr 18 00:32:19 EST 2001


In article <3ADD22AC.7AD6BD17 at wgn.net>, nienhuis at wgn.net says...
> You need to heat the PBS to 60 degrees C, and then it will all
> dissolve.
> 
> Bob
Thank you! Could you recommand me some good book or papers about 
immunohistochemical staning method? Thanks for your kindness.

Andrew


> 
> Andrew Huang wrote:
> > 
> > Dear Sir/Madam,
> >         Do you ever prepared for 4% paraformaldehyde in PBS buffer?
> > In immunohistochemical staining procedure, I found when I dissolved 4%
> > paraformaldehyde in 0.1M PBS buffer, there will be lots of
> > unsoluable, white materials in bottom. I'm so confused whether it is
> > natural results or my procedure is wrong. Could anybody tell me how and
> > why it is, please? Thank you!
> > 
> > Sincerely
> > 
> > Andrew
> 




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