Question for paraformaldehyde in PBS buffer
d8832005 at ccunix.ccu.edu.tw
Wed Apr 18 00:32:19 EST 2001
In article <3ADD22AC.7AD6BD17 at wgn.net>, nienhuis at wgn.net says...
> You need to heat the PBS to 60 degrees C, and then it will all
Thank you! Could you recommand me some good book or papers about
immunohistochemical staning method? Thanks for your kindness.
> Andrew Huang wrote:
> > Dear Sir/Madam,
> > Do you ever prepared for 4% paraformaldehyde in PBS buffer?
> > In immunohistochemical staining procedure, I found when I dissolved 4%
> > paraformaldehyde in 0.1M PBS buffer, there will be lots of
> > unsoluable, white materials in bottom. I'm so confused whether it is
> > natural results or my procedure is wrong. Could anybody tell me how and
> > why it is, please? Thank you!
> > Sincerely
> > Andrew
More information about the Neur-sci