Prepared questiones for 4% Paraformaldehyde in PBS

Sean Patterson seanpat at fmed2.uncu.edu.ar
Wed Apr 18 11:37:55 EST 2001


>Dear Sir/Madam,
>	Do you ever prepared to 4% paraformaldehyde in PBS buffer?
>When I prepared it, I found there were lots of unsoluble materials.
>I'm so confused whether it is ok. Could you tell me how to solve the
>problem or whether my procedure is wrong to be due to obtain unsoluble
>materials?
>
>Thanks for your help!
>
>Andrew

Try posting to bionet.molbio.methds-reagnts.

My recollection is of heating and pH-ing 4% paraformaldehyde in PBS 
in order to get it to dissolve.

-- 
Sean Patterson, Ph.D.
Catedra de Fisiologia, CC33
Facultad de Ciencias Medicas
Universidad Nacional de Cuyo
Mendoza, Argentina
Tel: (0261) 420-5115 ext.2684/2747
Fax: (0261) 449-4117
e-mail: seanpat at fmed2.uncu.edu.ar

---




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