Neonatal spinal prep

Richard Norman rsnorman at mediaone.net
Fri Aug 10 20:25:42 EST 2001


On Fri, 10 Aug 2001 16:31:52 -0500, "chakraba"
<samit at scrc.umanitoba.ca> wrote:

>Anyone with pointers to how one can remove a large amount of noise that one
>encounters when using neonatal rat whole spinal cord preparations?
>I have just started this.
>thanks

Is this "new" noise that you don't get with other preparations or do
you just have noise in your setup no matter what you are doing?

A standard thing to do is to forget about physiology -- replace all
you electrodes with equivalent resistors leaving the physical
arrangement of all leads and connections as close to normal as you
can.  Once you have clean "recordings" that way, you can then worry
about the preparation.

But make sure you really use appropriate high resistances to match the
actual electrode impedance of everything in your setup -- all
recording and stimulating and bath grounds.  Also make sure your
resistors are located approximately at the same physical location as
your elecrodes and that you keep everything else about the setup --
all lights, temperature controllers, etc. exactly the same.

Other than that, good luck.  Eliminating noise and interference is
more voodoo than science!





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