Neonatal spinal prep

chakraba samit at scrc.umanitoba.ca
Mon Aug 13 11:59:09 EST 2001


Thanks, Will try that.
Cheers
Samit

--

"Richard Norman" <rsnorman at mediaone.net> wrote in message
news:sv19ntgl25jcqa3tv1j17hctpg5mn3qn76 at 4ax.com...
> On Fri, 10 Aug 2001 16:31:52 -0500, "chakraba"
> <samit at scrc.umanitoba.ca> wrote:
>
> >Anyone with pointers to how one can remove a large amount of noise that
one
> >encounters when using neonatal rat whole spinal cord preparations?
> >I have just started this.
> >thanks
>
> Is this "new" noise that you don't get with other preparations or do
> you just have noise in your setup no matter what you are doing?
>
> A standard thing to do is to forget about physiology -- replace all
> you electrodes with equivalent resistors leaving the physical
> arrangement of all leads and connections as close to normal as you
> can.  Once you have clean "recordings" that way, you can then worry
> about the preparation.
>
> But make sure you really use appropriate high resistances to match the
> actual electrode impedance of everything in your setup -- all
> recording and stimulating and bath grounds.  Also make sure your
> resistors are located approximately at the same physical location as
> your elecrodes and that you keep everything else about the setup --
> all lights, temperature controllers, etc. exactly the same.
>
> Other than that, good luck.  Eliminating noise and interference is
> more voodoo than science!
>
>





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