Glial antibodies

[Henk Veldman]

Angie schreef:

> Does anyone know of a robust and fairly clean antibody to use for
> histochemistry that localizes to the cytoplasm of glia, preferably
> astrocytes, both activated and non activated?
>
> Thanks for any help!
>

Almost any producer of commercial antibodies will have one or more
preparation in its catalog against GFAP (glial fibrillary acidic
protein), both polyclonal and monoclonal. Try DAKO, Sigma, Chemicon,
well . . . any.
We are using the DAKO polyclonal antibody on sections of fixed rat brain
and on cultured cells to identify and locate astrocytes, much to our
satisfaction.

Henk

--
H. Veldman
Laboratory for Experimental Neurology (NMZ)
University Medical Center Utrecht (AZU)