need some advice in neuronal histochemistry

remini2 at earthlink.net remini2 at earthlink.net
Sat Jul 28 16:20:29 EST 2001


Hi everyone,

   I am currently working on the localization of glutaminase in rat
hippocampus. I use nitro blue to stain the enzymatic activity of
glutaminase. However, when I tried to embed the sample into the epon
resin, the stained purple clolor faded in the process of dehydration
(with ethanol), and the process of infiltration (with acetone). I
would like to know if there is any way to preserve the stains. Thanx!

Nick




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