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lv rui lvjie2001 at yahoo.com
Sat Oct 13 13:58:50 EST 2001


I have cultured some primary neurons from P5 rat
hippocampus, with Neural basal containing B27, bFGF,
glutamaxin as culture medium. On 3DIV, I found many
cluster cellualr islands. I think the density of cells
might be too high (400/mm2). Is anyone has good ideas
of avoiding cells cluster?

Jackl



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