Perforated Patch Problem

Justus Verhagen justus.verhagen at
Tue Sep 4 04:22:47 EST 2001

Alhtough not likely, but could your ampho have gone nearly completely
gone bad?

On 3 Sep 2001 20:27:23 -0700, mfireland at (Matthew Ireland)

>Dear Colleagues 
>Currently I am using perforated patch on neurons within the CNS.
>Recently, I have been experinceing a problem when I try to perforate a
>neuron, instead of a full perforation as you would expect, it only
>results in a partial perforation.
>I have tried different concentrations of Amphotericin B, different
>filling times and modified electrodes. All without much success.
>I would appreciate any suggestions
>With thanks,
>Matthew Ireland
>University of Queensland

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