DRG neurons' markers

outofbodyeperience shadow at oob.com
Fri Sep 28 13:17:33 EST 2001


How about trying rat DRGs or anti VR1 & VR2 distinguish between small
and large neurons. I believe they are commercially available

celiacruz at netc.pt wrote:

> Hi everyone!
>
> I am a portuguese Ph.D student and I'm trying
> to stain big and medium-sized DRG neurons with
> NF200 (N52) antibody using the ABC technique.
> The problem is that the antibody is made in
> mouse and I'm using mouse tissue. I have already
> bought the Mouse on Mouse Kit from Vector but
> I am still having problems. I first tryed diluting
> the antibody 1/500 and 1/1000. Since I got
> a lot of background, I tryed diluting it in
> PBST and incubating at 4ºC. Still bad. I've
> tryed blocking with glycine and normal goat
> serum. Worse!
>
> I'm really running out of ideas. Can anyone
> give me a hint (as soon as possible, please)on
> how to proceed?
>
> Many thanks to all,
> Célia




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