patch-clamp recordings

Ryan jh_kix at
Mon Sep 23 09:11:08 EST 2002

I´m trying to understand an article that involves the use of
whole-cell slice patch-clamp recordings. Could anyone explain or
direct me to relevant resources on potential pitfalls that occur with
prolonged use of this technique. Also, I´m not sure if the following
makes sense to me (esp the meaning of perforated in this context): we
measured whole-cell currents elicited in medium spiny neurons after
3-7 days in culture in perforated patch mode to prevent intracellular
perfusion from the recording electrode.

- Ryan

P.S. If anyone interested in neuropharmacology is willing to offer
guidance on other related topics, I would be happy to hear from you
(cel at

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