Kinetics of DNQX and bicuculline?

tomte tehgabriel at
Tue Jul 6 06:40:54 EST 2004

I want to do a set of patch-clamp experiments with combined blockade
of synaptic AMPA and GABA currents. For experimental reasons I'll have
to do all recordings within a timespace of only a few minutes (5' up
to max 10').
Because I have rather few experience with DNQX/bic. I find it hard to
forecast if this plan is feasible. So here is my question:
   How long does it take until DNQX and bicuculline respectively reach
their full effect on synaptic transmission? And following wash-out:
how long does it take until their effect is attenuated in such a
degree that synaptic activity may be comparable to the control
situation before application of blockers?

Every comment is grateful appreciated!

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