staining for dendrite and axon
m.kirkcaldie at removethis.unsw.edu.au
Mon Mar 15 22:03:45 EST 2004
In article <Xns94AE9EE50B9BABilZ0rhotmailcom at 22.214.171.124>,
BilZ0r <BilZ0r at TAKETHISOUThotmail.com> wrote:
> MAP-2 yes.
> Tau... I don't know.
> myang3 at uiuc.edu (Mong-Lin Yang) wrote in news:4f849c85.0403101802.1bddfb09
> > Hi,
> > Does anyone know a good way of descerning dendrite or axon by
> > staining? Is it correct that Map-2 antibody stains only dendrite and
> > Tau antibody stains only axon? thanks!
MAP-2 is a definitive dendrite label; postsynaptic proteins are also
useful but less ubiquitous. Axons are tougher - I don't think tau is
present in every axon (it's also a MAP, by the way). I have heard of
the use of an antibody to alpha-acetylated tubulin as a reliable axon
marker - but I have no experience with it. You should look that one up
and see if it's suitable.
(PS: Did you know that if you destabilise the cytoskeleton of a
developing dendrite it turns into an axon? So you can make neurons with
multiple axons using appropriate manipulations in culture. Just a bit
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