increase in electrode resistance
kpaulc at [remove]earthlink.net
Thu Mar 25 05:26:09 EST 2004
"Elizabeth Thomas" <ethomas at ulg.ac.be> wrote in message
news:1987c6fa.0403240256.c32ceee at posting.google.com...
> I am currently doing sharp electrode current clamp recordings on
> thalamic slices. I have an intermittent problem (but it occurs with
> high frequency) with the recordings that has been bothering me. When
> I start out with the electrodes in the bath, I can start at very
> reasonable resistances like 90-120 Mohms. But once I penetrate a
> neuron and stablize it with hyperpolarizing currents - the resistance
> climbs up very high to more than 300 MOhms. At this point it is no
> longer possible for me to continue the experiment.
> This problem only occurs after I have penetrated a neuron. The
> resistance is stable even when I leave the electrode in the bath for a
> long time. I also have the impression that the problem is very
> reduced during lunch hour.
Even though it's not explicitely Neuroscience,
this 'out-to-lunch' 'differential' is =Worthy= of
Study, and I hope you'll get to the bottom it,
k. p. collins
> Has anyone had this problem before? Any suggestions?
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