increase in electrode resistance

ken kpaulc at [remove]
Thu Mar 25 05:26:09 EST 2004

"Elizabeth Thomas" <ethomas at> wrote in message
news:1987c6fa.0403240256.c32ceee at
> I am currently doing sharp electrode current clamp recordings on
> thalamic slices.  I have an intermittent problem (but it occurs with
> high frequency) with the recordings that has been bothering me.  When
> I start out with the electrodes in the bath, I can start at very
> reasonable resistances like 90-120 Mohms.  But once I penetrate a
> neuron and stablize it with hyperpolarizing currents - the resistance
> climbs up very high to more than 300 MOhms.  At this point it is no
> longer possible for me to continue the experiment.
> This problem only occurs after I have penetrated a neuron.  The
> resistance is stable even when I leave the electrode in the bath for a
> long time.  I also have the impression that the problem is very
> reduced during lunch hour.

Even though it's not explicitely Neuroscience,
this 'out-to-lunch' 'differential' is =Worthy= of
Study, and I hope you'll get to the bottom it,

k. p. collins

> Has anyone had this problem before?  Any suggestions?
> Sincerely
> Elizabeth

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