Temperature and voltage clamp

r norman rsn_ at _comcast.net
Mon Nov 8 08:28:15 EST 2004


On Mon, 08 Nov 2004 07:53:47 GMT, BilZ0r
<BilZ0r at TAKETHISOUThotmail.com> wrote:

>Hi,
>I was talking to a colleague today, who primary studies antiepileptics with 
>voltage clamp on voltage sensitive sodium channels expressed in X. Oocytes. 
>He told me he does all of his work, I believe, at 15ºC... Does that sound 
>right?
>
>Why would he use such a low temperature? So he could see any effects of 
>gating more readily? Any ideas?

Why do you think 15 C is a "low" temperature?  Remember, the work is
done on Xenopus cells, not mammalian.  Xenopus ordinarily lives at
temperatures of 15 to 25 C.  Preparations do last longer and remain in
better conditions at cooler temperatures.





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