> i'm interested in anti-phosphotyrosine or serine antibodies. has
> anybody used them to look at membrane-associated proteins from
> neuronal growth cones ? if so, i would like like to get references
> is it possible to use these abs in in situ immunolocalization ?
I would direct you to a couple of recent articles on this subject:
Wu, D.-Y. and Goldberg, D.J. (1993) Regulated tyrosine
phosphorylation at the tips of growth cone filopodia.
J. Cell Biol. 123:653-664. (Nov. 1993)
Atashi, J.R. et al. (1992) Neural cell adhesion molecules
modulate tyrosine phosphorylation of tubulin in nerve
growth cone membranes. Neuron 8:831-842. (May 1992)
Note that the tubulin in the Atashi et al. study is *membrane-
associated* (pretty interesting). In fact, some of the co-authors
of this study had previously reported that tubulin (alpha and
beta) is phosphorylated on tyrosine residues by pp60c-src
in growth cone membranes (J. Cell Biol. 111:1959-1970).
The Atashi et al. paper reports that tubulins are the major
phosphoproteins labelled by radioactive ATP in untreated
growth-cone enriched membranes.
> i know that there are tons of phosphoproteins in a cell but maybe
> there are certain stringent adsorption conditions that would give
> very specific immunolocalization signals ?
Wu and Goldberg seem to have accomplished this.
Steve Matheson Program in Neuroscience University of Arizona
sfm at neurobio.arizona.edu