I have some experience with perforated patch-clamp. For Nystatin you
should simply to take care to work in darkness, when possible (it is
UV-sensitive...). Stock solution should be stored (-20ºC) in those color
eppendorf tubes or wrapped with Aluminum foil, to keep them away from
the UV rays....
I could give you more advices, if you tell me exactly which kind of
cells are you trying to patch (slices or cell-culture?).
I personally find Anphotericin B better for perforated patching! :P
bilz0r at hotmail.com wrote:
>> I've recently got in some Fluka nystatin from sigma:
>http://www.sigmaaldrich.com/catalog/search/ProductDetail/FLUKA/74721>> ..for perforated patching. Now while I understand how to use nystatin
> in theory, the actual practicalities escape me. Specifically, it says
> on the side of the bottle, that it is UV sensitive, and that I need to
> store it under argon! I don't think there is any argon within a mile
> from my lab, what am I supposed to do when I make up my portion of DMSO
> stock? Or is it one of those kinda "in a perfect world, you'd store it
> under argon, but in reality, no one actually does this" kinda warnings?
>> Anyone know?