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[Neuroscience] Re: Nystatin for perforated patches

bilz0r at hotmail.com bilz0r at hotmail.com
Tue Jun 28 19:05:30 EST 2005


Thanks for replying.

I'm probably going to be patching pyramdal cells in the cortex (blind),
or Nigral cells (DIC visualization) in mouse brain slices.



SJM Guzman wrote:
> Hi there!
>
> I have some experience with perforated patch-clamp. For Nystatin you
> should simply to take care to work in darkness, when possible (it is
> UV-sensitive...). Stock solution should be stored (-20ºC) in those color
> eppendorf tubes or wrapped with Aluminum foil, to keep them away from
> the UV rays....
>
> I could give you more advices, if you tell me exactly which kind of
> cells are you trying to patch (slices or cell-culture?).
>
> I personally find Anphotericin B better for perforated patching! :P
>
> Regards!
>
>
> bilz0r at hotmail.com wrote:
> > Hi,
> >
> > I've recently got in some Fluka nystatin from sigma:
> > http://www.sigmaaldrich.com/catalog/search/ProductDetail/FLUKA/74721
> >
> > ..for perforated patching. Now while I understand how to use nystatin
> > in theory, the actual practicalities escape me. Specifically, it says
> > on the side of the bottle, that it is UV sensitive, and that I need to
> > store it under argon! I don't think there is any argon within a mile
> > from my lab, what am I supposed to do when I make up my portion of DMSO
> > stock? Or is it one of those kinda "in a perfect world, you'd store it
> > under argon, but in reality, no one actually does this" kinda warnings?
> > 
> > Anyone know?
> > 
> > Thanks
> > 
> 
> 
> -- 
> ÿþS



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