Thanks for replying.
I'm probably going to be patching pyramdal cells in the cortex (blind),
or Nigral cells (DIC visualization) in mouse brain slices.
SJM Guzman wrote:
> Hi there!
>> I have some experience with perforated patch-clamp. For Nystatin you
> should simply to take care to work in darkness, when possible (it is
> UV-sensitive...). Stock solution should be stored (-20ºC) in those color
> eppendorf tubes or wrapped with Aluminum foil, to keep them away from
> the UV rays....
>> I could give you more advices, if you tell me exactly which kind of
> cells are you trying to patch (slices or cell-culture?).
>> I personally find Anphotericin B better for perforated patching! :P
>> Regards!
>>>bilz0r at hotmail.com wrote:
> > Hi,
> >
> > I've recently got in some Fluka nystatin from sigma:
> > http://www.sigmaaldrich.com/catalog/search/ProductDetail/FLUKA/74721> >
> > ..for perforated patching. Now while I understand how to use nystatin
> > in theory, the actual practicalities escape me. Specifically, it says
> > on the side of the bottle, that it is UV sensitive, and that I need to
> > store it under argon! I don't think there is any argon within a mile
> > from my lab, what am I supposed to do when I make up my portion of DMSO
> > stock? Or is it one of those kinda "in a perfect world, you'd store it
> > under argon, but in reality, no one actually does this" kinda warnings?
> >
> > Anyone know?
> >
> > Thanks
> >
>>> --
> ÿþS
