Staining of neurons: biocytine vs. lucifer yellow?

tehgabriel at web.de tehgabriel at web.de
Thu May 12 05:33:34 EST 2005


Hi!
So far i have no experience with staining of cells e.g. cortical
neurons. For a new set of experiments we thought it might be useful to
start with this in order to discriminate certain morphological criteria
(some dendritic projections, general shape of dendritic tree etc.). It
seems that the most common methods for staining are via biocytine or
via lucifer yellow. What are the advantages/disadvantages of these two
compounds?
So far as i know, lucifer yellow is very easy to use, requires no
histochemical preparation but does not stain fine dendritic structures.
Biocytine, on the other hand, allows a finer resolution but requires
some histochemics after the recording.
What do you think/is your experience?

Every tip or comment is appreciated.
Cheers,
Thomas




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