Staining of neurons: biocytine vs. lucifer yellow?

Doreen Milius doreen.milius at medizin-uni-leipzig.de
Tue May 17 05:37:30 EST 2005


I think what you can see in this picture is the dendritic arbourization.
With this kind of staining it is not possible to see the axon projections.

Regards!





> I guess you've seen lots of examples of the labelling already, but here's
a
> web site with a nice confocal pic of a lucifer yellow filled granule
neuron
> from the rat.  You can even follow the axon for quite some distance in
their
> paper I think.
>
>
> http://www.utoronto.ca/neurosci/main_page_picture_page.html
>
>
> <tehgabriel at web.de> wrote in message
> news:1115894014.112677.152940 at f14g2000cwb.googlegroups.com...
> > Hi!
> > So far i have no experience with staining of cells e.g. cortical
> > neurons. For a new set of experiments we thought it might be useful to
> > start with this in order to discriminate certain morphological criteria
> > (some dendritic projections, general shape of dendritic tree etc.). It
> > seems that the most common methods for staining are via biocytine or
> > via lucifer yellow. What are the advantages/disadvantages of these two
> > compounds?
> > So far as i know, lucifer yellow is very easy to use, requires no
> > histochemical preparation but does not stain fine dendritic structures.
> > Biocytine, on the other hand, allows a finer resolution but requires
> > some histochemics after the recording.
> > What do you think/is your experience?
> >
> > Every tip or comment is appreciated.
> > Cheers,
> > Thomas
> >
>
>





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