Staining of neurons: biocytine vs. lucifer yellow?
darwin at beagle.net
Tue May 17 23:14:52 EST 2005
Actually, you can see the axon. I think they cut it off in this picture
though. From the same series of experiments from this lab, they filled
granule neurons in the dentate gyrus with Lucifer Yellow while they were
doing patch recordings and you can easily follow the axon through the hilus
to its terminal on CA3 pyramidal neurons. They were also able to see the
spines on the dendrites quite clearly.
"Doreen Milius" <doreen.milius at medizin-uni-leipzig.de> wrote in message
news:d6chhf$ta$1 at news1.uni-leipzig.de...
> I think what you can see in this picture is the dendritic arbourization.
> With this kind of staining it is not possible to see the axon projections.
> > I guess you've seen lots of examples of the labelling already, but
> > web site with a nice confocal pic of a lucifer yellow filled granule
> > from the rat. You can even follow the axon for quite some distance in
> > paper I think.
> > http://www.utoronto.ca/neurosci/main_page_picture_page.html
> > <tehgabriel at web.de> wrote in message
> > news:1115894014.112677.152940 at f14g2000cwb.googlegroups.com...
> > > Hi!
> > > So far i have no experience with staining of cells e.g. cortical
> > > neurons. For a new set of experiments we thought it might be useful to
> > > start with this in order to discriminate certain morphological
> > > (some dendritic projections, general shape of dendritic tree etc.). It
> > > seems that the most common methods for staining are via biocytine or
> > > via lucifer yellow. What are the advantages/disadvantages of these two
> > > compounds?
> > > So far as i know, lucifer yellow is very easy to use, requires no
> > > histochemical preparation but does not stain fine dendritic
> > > Biocytine, on the other hand, allows a finer resolution but requires
> > > some histochemics after the recording.
> > > What do you think/is your experience?
> > >
> > > Every tip or comment is appreciated.
> > > Cheers,
> > > Thomas
> > >
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