[Neuroscience] whole cell recording from dispersed neurons

drb mjbeyak at yahoo.ca
Wed Apr 5 13:46:55 EST 2006


We have fairly extensive experience in recording from acutely dispersed
sensory neurons (1.e. 1-2 days after dispersion), and have used almost
exactly the same solutions as yourself (and there is also tons of literature
using these solutions as well) so I doubt that is the problem. Bubbling with
O2 should not be necessary. Some people warm the extracellular solution in
the incubator before transfer into this solution as sometimes the neurons
will lift off the coverslip when suddenly plunged into cold room temp
solution. 
When you say the cells are dead what do you mean? Are the cells lysed? Do
they lift off the coverslip? Or do they just not have good healthy resting
membrane potentials? What is the evidence that they are vaible to begin
with? In most people's experience the problems lie in the culturing /
dissociation process, but without knowing what you mean by dead cells its
difficult to provide any more advice. 
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