[Neuroscience] Re: Neur-sci Digest, Vol 11, Issue 6

Bill connelly.bill at gmail.com
Wed Apr 5 19:35:50 EST 2006


That shouldn't be a problem, our primary cultures are grown in
neurobasal media too and recorded from in a very similar (room
temperature) aCSF.

So they all die, as soon as you take them out of the incubator? Or do
they die only after you patch them. If it's the later, I suspect your
electrode, pipette solution, or break in technique. If it is the
former, then I have no idea. My only suggestion is to half, or quater
your calcium chloride, and drop your KCl down to 2.5mM. Oh, and try
30mM glucose.

I know they sound like silly changes, but anything is possible?

(my cell culture extracellular saline (mM) NaCl 142, KCl 2.5, CaCl,
2-0.5, MgCl 2, HEPES 5, Glucose 30. pH 7.4)



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