[Neuroscience] Biocytin and double-labelling in EM

Linda Hassinger lhassinger at mclean.harvard.edu
Fri Mar 3 12:40:41 EST 2006


Hi, I happened to pick up this message from you while looking for something 
else.  I double label  rat  1-Gaba  with gold  2-fibers dab 
biocytin.  There are several tricks that I employ to get these to work.  I 
try to keep the fiber staining light to be able to see the synapses.  I 
cryo protect the sections and freeze thaw  the sections packed in baby 
powder to improve the Gaba staining and preserve the ultrastructure. Yes, 
baby powder.  It works!  I now enhance the gold with silver.  I can give 
you more details if you are interested.
Regards,
Linda Hassinger



Any information, including protected health information (PHI), transmitted
 in this email is intended only for the person or entity to which it is
addressed and may contain information that is privileged, confidential and or
exempt from disclosure under applicable Federal or State law. Any review,
retransmission, dissemination or other use of or taking of any action in
reliance upon, protected health information (PHI) by persons or entities other
than the intended recipient is prohibited. If you received this email in error,
please contact the sender and delete the material from any computer.



More information about the Neur-sci mailing list