[Neuroscience] Re: In vitro chambers - custom fabrication facilities
(by connelly.bill At gmail.com)
Sun Nov 12 18:46:09 EST 2006
Why pay someone to do it, when you can do it yourself. Making things is
the best bit.
Get some perspex, cut a hole the size you need for your brain slice.
Glue a ITO coated coverslip heater onto to cover the whole (if you want
to heat your brain slice); or just a coverslip if you'll work a room
temperature. Use ether a circular saw, or a file to make a trench in
and out of the chamber you've made (best to do this before you glue
anything in). Make a deeper well on the way out (usually by glueing on
another piece of perspex, then drilling through, to make the well. Glue
to teflon tubing on, for the inlet (remember to use silicon glue; water
proof and does not leech anything once dry). Cut the thing to the right
size to fit on your microscope stage, and you're done.
That's just one way of many.
But if you're sure you want to buy a chamber,
http://www.biosciencetools.com/catalog/Temperature.htm makes okay ones
RYK Pun wrote:
> To whom this may concern,
> I came across a web-page indicating facilities available to
> manufacture customized chamber for brain slice recording. I'm writing
> to find out whether those facilities are still open and how I can
> contact the people running the facilities. I'd appreciate if you
> could provide me with any information related to this. Thank you.
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