[Neuroscience] Re: siRNA transfection of primary neurons

sirshane13 via neur-sci%40net.bio.net (by sirshane13 At gmail.com)
Wed Nov 15 18:29:38 EST 2006


Hello,

My apologies in advance since this is not my area of expertise, however
a paper was just brought to my attention this evening that was
published this year concerning something similar to this in Eur. J.
Neuro. (2006). I do not know to what extent it is applicable to your
work, but it might provide some insight if you are not already familiar
with it.

See Chen et al in Vol. 24.

O'Hare wrote:
> Has anybody managed to successfully transfect primary cultures of
> cerebellar granule neurons with short double stranded siRNA?  If so,
> what transfection reagent did you use and how much RNA and transfection
> reagent needs to be combined to achieve optimal transfection?  Any
> additional details that could be provided would also be greatly
> appreciated.  Thank you.
>
>
>
> -------------------------------------------------------------------
>
> Michael J. O'Hare, Ph.D.
>
> Tel: (613) 993-4294 | Fax: (613) 941-4475 | email: michael.ohare At nrc.ca
>
>
>
> Institute for Biological Sciences
>
> National Research Council of Canada
>
> 1200 Montreal Road, Building M-54
>
> Ottawa, Ontario K1A 0R6, Canada
> 
> -------------------------------------------------------------------



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