[Neuroscience] Re: Biocytin fills?

Christian Wilms via neur-sci%40net.bio.net (by usenet02 At out-of-phase.de)
Thu Nov 16 05:15:54 EST 2006


Hi Bill,

I've successfully used Biocytin tracing in 200 micron slices without
resectioning. I had 0.5% Neurobiotin (Vector Labs) in my pipette
soultion. After recording I carefully removed the pipette from the cell,
making sure not to destroy the cell. The slice was then transferred
immediately to a 0.4% paraformaldehyd solution and "incubated" at 4°C
for several days. The rest was "by the book" staining.

Hope that is of some help,

Christian


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