[Neuroscience] Re: How to make brain slices with cortico striatal connections?

Bill via neur-sci%40net.bio.net (by connelly.bill from gmail.com)
Sat Apr 28 22:02:22 EST 2007


This is quite simple, it will even work on rats. Completely coronal
slices are unlikely to get you the result.
The key is to cut the para-coronal slices on a at ~45 degrees to the
coronal plane rotated through the horizontal axis.

Ah, that's hard to explain. Like this.
http://www.ilikethings.net/corticostriatal.JPG

See:
Arbuthnott GW, MacLeod N, and Rutherford A. The rat cortico-striatal
pathway in vitro. J Physiol (Lond) 367: 102P, 1985.

On Apr 24, 8:08 pm, tomte <tehgabr... from web.de> wrote:
> Hi!
> We are trying to figure out how to make brain slices (in mice) where
> connections between striatum and cortex are preserved? Any idea?
>
> So far we tried coronal slices (refering to what is stated in most of
> the literature) but for some reason we can't find any neurons in
> striatum and cortex that are synaptically connected.
> Can it be that other cutting planes work better? Might cutting in a
> certain angle be necessary to obtain functional connections?
>
> Any ideas are apreciated!
>
> Regards,
> Thomas




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