[Neuroscience] Re: How to make brain slices with cortico striatal
(by connelly.bill from gmail.com)
Sat Apr 28 22:02:22 EST 2007
This is quite simple, it will even work on rats. Completely coronal
slices are unlikely to get you the result.
The key is to cut the para-coronal slices on a at ~45 degrees to the
coronal plane rotated through the horizontal axis.
Ah, that's hard to explain. Like this.
Arbuthnott GW, MacLeod N, and Rutherford A. The rat cortico-striatal
pathway in vitro. J Physiol (Lond) 367: 102P, 1985.
On Apr 24, 8:08 pm, tomte <tehgabr... from web.de> wrote:
> We are trying to figure out how to make brain slices (in mice) where
> connections between striatum and cortex are preserved? Any idea?
> So far we tried coronal slices (refering to what is stated in most of
> the literature) but for some reason we can't find any neurons in
> striatum and cortex that are synaptically connected.
> Can it be that other cutting planes work better? Might cutting in a
> certain angle be necessary to obtain functional connections?
> Any ideas are apreciated!
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