[Neuroscience] Re: Does TTX degrade?
(by connelly.bill from gmail.com)
Thu Aug 2 16:51:08 EST 2007
I know that TTX degrades at 4 degrees in saline overnight.
I suppose the proof of the pudding is in the tasting; find someone
with some cultured neurons, or some other electrophysiological
apparatus, if a hand-full of nanomolar (10-50) of TTX is enough to
block action-potential generation, then it's still good.
Our lab regularly keeps TTX at -20 for a year, with no observable loss
of potency, but I don't know about stretching that to over a decade
On Aug 1, 3:51 pm, "Melinda T. Owens" <owens... from phy.ucsf.edu> wrote:
> I would like to begin a research project with TTX, but the supply my
> lab has is from 1994. (It's been dissolved in saline, aliquoted, and
> continually kept frozen at -20.) My professor is really cheap, so I
> don't want to buy more if I don't have to. Does TTX degrade over
> time? How long can I keep it before I should be worried about its
> losing efficacy?
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