[Neuroscience] Re: In vivo whole-cell recording

Benjamin via neur-sci%40net.bio.net (by Benjamin At verizon.net)
Fri Feb 2 10:47:55 EST 2007


"Hyunchul Lee" <hlee At medsci.usyd.edu.au> wrote in message 
news:mailman.1030.1170380573.19683.neur-sci At net.bio.net...
> We've started doing in vivo whole cell recording in an anaesthetized 
> mouse.
> Contrary to what we first thought, stability wasn't as big an issue for 
> us.
> However, it does seem a lot harder to patch on than in slices...
> My boss tells me it's just about getting used to it, but I'm not so sure 
> that's all it is.
>
> So the way I patch is apply positive pressure going into the bath/brain, 
> enter the slice/brain and apply positive pressure again,
> then go about looking for my cell.  The time that we did get cells, I 
> remember that we applied a lot of suction to patch on.
>
> Say, does anyone know if intracranial pressure could be the culprit?
> If so, some papers say they open the cisterna magna to relieve 
> intracranial pressure.
> Would that do it for us?
>
> Thanks.

There are innate ionic conductances
in vivo, and it's important to allow them
to occur as naturally as possible if re-
sults are to be meaningful.

This is a difficult problem that, if I were
at the bench, I'd try to get a handle on
by using tracable ionic isotopes via an
appropriate scanning technique.

This's a different problem than single-
cell recording, but, in the end, it will
yield the 'same' information, except
much more of it.

Cheers, and good luck in your efforts.

ken 




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