[Neuroscience] Re: Ideas for getting a Sutter to pull reasonable pipettes?

Imre Vida via neur-sci%40net.bio.net (by I.Vida from bio.gla.ac.uk)
Sat Jul 7 07:00:11 EST 2007


On Fri, Jul 06, 2007 at 08:07:57PM -0700, Bill wrote:
> So the first pull goes very close to pulling the capillary in half,
> but just not? and the second finishes it off?
set pull=0, reduce heat and velocity 
keep velocity as low as possible

> How many stages do other people use? I saw one guy reporting a 7 stage
> pull in a paper.
we usually have 5-6 cycles and start with a one-liner
you can later increase the number of lines and refine 
settings 


imre



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