[Neuroscience] Stationary Noise analysis for single-channel info?

Bill via neur-sci%40net.bio.net (by connelly.bill from gmail.com)
Tue Jun 19 23:50:01 EST 2007


Hi,

If I've got a standing background noise in a whole-cell voltage clamp
experiment that I know is mediated by an ion channel (it goes away in
the presence of all the right drugs and is enhanced by the right drugs
etc), I am sure I can get some information on the single channel
conductances that are making this noise.

I can find several papers that say things like "Given Variance^2 =
i^2npo(1-po) we calculated a lorentzian function and found the single
channel conductance".

How do I ACTUALLY do this? Anyone know? I'm sure I could get a graph
program to fit a lorentzian function to a curve, if I could calculate
the right curve in the first place, which is a spectral density
function, which plots A^2/Hz vs Hz.

Does anyone have any idea what I'm talking about? (I certainly don't).
Can anyone offer some rough tips on what I need to do? I have access
to CED software, MiniAnalysis and John Dempsters WinWCP package.



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