[Neuroscience] Biocytin labeling in adult brain slices

[Karthik Rajasekaran]

Hi
I have only limited success (approx. 50%) with biocytin labelling of neurons 
from which I record whole cell currents from thalamic relay neurons. The 
protocol that I use is fairly standard in which singal is amplified by 
incubating the sections in ABC and then reacted with DAB. The slices are 
obtained 8-9 month old rats, and often those that present with extensive 
gliosis....though I do not see any reason why that would affect it as long 
as I get my cell to record...  Any advice / suggestions will be greatly 
appreciated. Thank you!
Karthik