[Neuroscience] Increasing series resistance

[Han Xu]

Hi All,

I recently study long-term synaptic plasticity in neocortex with  
patch clamp recording. The biggest problem I am facing is that series  
resistance is always going up over time, which decreases synaptic  
response amplitude. As a result, it is impossible to define the real  
potentiation or depression after conditioning stimuli. One thing I  
doubt is the internal pipette solution with an osmolarity of 271  
mOsm, which is low compared with the value mentioned in literature in  
the range of 290-310 mOsm. Is there anybody has experience on this  
problem? I will be really appreciated if somebody can give me any  
suggestions.

Best,
Han