[Neuroscience] Re: Increasing series resistance

mathneuro via neur-sci%40net.bio.net (by mathneuro from gmail.com)
Mon Oct 22 21:36:29 EST 2007

On Oct 22, 2:59 pm, Han Xu <h... from cns.nyu.edu> wrote:
> Hi All,
> I recently study long-term synaptic plasticity in neocortex with  
> patch clamp recording. The biggest problem I am facing is that series  
> resistance is always going up over time, which decreases synaptic  
> response amplitude. As a result, it is impossible to define the real  
> potentiation or depression after conditioning stimuli. One thing I  
> doubt is the internal pipette solution with an osmolarity of 271  
> mOsm, which is low compared with the value mentioned in literature in  
> the range of 290-310 mOsm. Is there anybody has experience on this  
> problem? I will be really appreciated if somebody can give me any  
> suggestions.
> Best,
> Han

Can you explain why the series resistance (presumably of the membrane
but unclear) is increasing? Or if that is unknown, how it is being

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