[Neuroscience] Re: Technical question: dissociated cells
(by jalegris from sympatico.ca)
Sat Apr 26 17:42:28 EST 2008
On Apr 24, 2:15 am, christinela... from hotmail.com wrote:
> Hi, I'm wondering if anyone here has done electrophysiology on acutely
> dissociated neurons? I've found several methods in the literature and
> have had a couple of attempts with adult rat brain. The cells come out
> looking bright and round and alive, but unfortunately I can't make
> them stick to glass chips, with or without poly-D-lysine coating. I
> need them to stick to something in order to patch them.
> Any suggestions?
How about a micropipette under low negative pressure.
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