[Neuroscience] Re: Cuttting solutions for electrophysiology
(by nin from neurohost.org)
Mon Dec 1 05:45:55 EST 2008
Thank you for the tips, I will try 3mM ascorbate late this week and possibly
glycerol. I will let you know in every detail.
And yes, the reason why not so many researchers are working on CA3 in
slices is due to the difficulty of getting nice cells. I am preparing a
document to describe the preparation of CA3 neurons in every little detail,
and I will send you when it's finished.
Thank you very much!
On 2008-12-01, Bill <connelly.bill from gmail.com> wrote:
> Mmm, there is something funny about CA3 cells, they've got to be one
> of the most delicate set of cells.
> The reason I reccomend the ascorbate is because of its important role
> in preventing the edema that occurs in slices. Check out:
> Ascorbate inhibits edema in brain slices.
> Brahma B, Forman RE, Stewart EE, Nicholson C, Rice ME.
> J Neurochem. 2000 Mar;74(3):1263-70
> I've really noticed an improvement in the patchability of small cells
> if the slices are constantly incubated in ascorbate.
> I wonder about 3mM myo-inositol for similar reasons, though I've never
> tried that.
> Ah well, let me know how things go, I'm curious about the glycerol, I
> saw that paper, and it seemed a bit... amazing.
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