[Neuroscience] Re: does spermine impede patch clamping neurons?

Bill via neur-sci%40net.bio.net (by connelly.bill from gmail.com)
Sat Dec 20 21:44:58 EST 2008


This wont be much help Karthik, but no, I've patched with spermine in
my pipette for 3 weeks, and I didn't find it effected initial seal
quality, or longevity of recordings.

On Dec 10, 8:36 am, Karthik <rajasekaran.kart... from gmail.com> wrote:
> Hi, I am trying to record rectification of AMPA currents, and am
> having problems with the quality of patch clamping if I add 100 microM
> of spermine to my pipette solution. I tried by first back filling my
> pipette with the regular internal and then filling with spermine
> containing internal, in which case the quality of seal degrades within
> a few minutes of patching the cell. Would appreciate any input on this
> problem. Thanks a lot!
> Karthik



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