[Neuroscience] Re: Brain stem slices? Agar?
(by Matthew.Kirkcaldie from removethis.utas.andthis.edu.au)
Tue Feb 26 22:33:00 EST 2008
#1, but then try agar if it breaks apart. The pyramidal tracts will be
your principal physical barrier.
As for the agar - dissolve the agar in very hot water as usual, draw
about 5ml into a 20ml syringe, aspirate about 10ml of air through it (or
run cold water around the outside, tip it up to push the air out,
repeat, until it's just warm on your skin (like testing a baby's
bottle). At that point you have about 2-3 minutes before it sets. About
a teaspoon per 100ml saline gives a consistency like brain tissue.
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