[Neuroscience] Re: Horse Radish Peroxidase -DAB Reaction

Bill via neur-sci%40net.bio.net (by connelly.bill from gmail.com)
Sat Jun 14 20:36:59 EST 2008


Don't worry. I found the answer. H2O2 reacts, which releases O2, which
reacts with the DAB.

On Jun 15, 11:20 am, Bill <connelly.b... from gmail.com> wrote:
> Hi,
>
> Does the chromogenic reaction of HRP with DAB actually require H2O2 at
> the same time? That is to say, in an isolated system (with no
> endogenous peroxisdases to complicate things), does HRP + DAB = BROWN
> require H2O2? If so, how is that reaction actually happening, i.e.
> what is being released or consumed by the HRP such that DAB reacts?
>
> Thanks



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