[Neuroscience] rat brain perfusion and fixation
Mary P. Brownson
(by mpb1211 from uwyo.edu)
Mon Jun 30 17:16:54 EST 2008
I am working on perfusion and fixation of rat brains for the purpose of
Fluoro-Jade staining after the brain has been sectioned with a cryostat.
I am having problems with the brain sections looking like "Swiss
cheese". Has anyone else had this problem? What can I do about this
problem? I am using 4% paraformaldehyde in PBS. Any info/hints will be
Mary Brownson, Ph.D.
School of Pharmacy
University of Wyoming
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