[Neuroscience] Re: Technical question: dissociated cells
anjum_zfr from yahoo.com
(by anjum_zfr from yahoo.com)
Sat May 10 09:23:31 EST 2008
we are using newborn hipppocampal neurons for patching at JCSMR, ANU,
Canberra. they grow well and stick to glass coverslipes as well.
Though I am new student here and learning patching here. I don't have
the references with me rightnow but i can send you them if you wish.
> On Apr 24, 2:15�am, christinela... from hotmail.com wrote:
> > Hi, I'm wondering if anyone here has done electrophysiology on acutely
> > dissociated neurons? I've found several methods in the literature and
> > have had a couple of attempts with adult rat brain. The cells come out
> > looking bright and round and alive, but unfortunately I can't make
> > them stick to glass chips, with or without poly-D-lysine coating. I
> > need them to stick to something in order to patch them.
> > Any suggestions?
> How about a micropipette under low negative pressure.
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