[Neuroscience] problems with dissociated cells & Ca2+ current recording

Sibylle Guggenmoos-Schreyer via neur-sci%40net.bio.net (by sibylle.guggenmoos from uniklinik-freiburg.de)
Mon Apr 20 03:30:52 EST 2009


I am trying to record Ca2+ currents (whole-cell) from acutely  
dissociated hippocampal CA1 neurons (7-10 day-old rats and mice).  
However, the cells only survive a couple of minutes after having  
established the patch. I wonder if this is a dissociating or a bath  
solution problem, as I can't bubble the bath solution with carbogene  
(it becomes too acidic). I tried recording Ca currents in acute slices  
of very young rats before, and those currents were stable but very  
difficult to influence with various substances which is why I think  
the bath solution might be the cause of trouble.
So, if anyone's got either a good bath solution recipe or a  
dissociating method or any other idea - I'd be so grateful!
Sibylle




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