[Neuroscience] problems with dissociated cells & Ca2+ current
(by sibylle.guggenmoos from uniklinik-freiburg.de)
Mon Apr 20 03:30:52 EST 2009
I am trying to record Ca2+ currents (whole-cell) from acutely
dissociated hippocampal CA1 neurons (7-10 day-old rats and mice).
However, the cells only survive a couple of minutes after having
established the patch. I wonder if this is a dissociating or a bath
solution problem, as I can't bubble the bath solution with carbogene
(it becomes too acidic). I tried recording Ca currents in acute slices
of very young rats before, and those currents were stable but very
difficult to influence with various substances which is why I think
the bath solution might be the cause of trouble.
So, if anyone's got either a good bath solution recipe or a
dissociating method or any other idea - I'd be so grateful!
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