[Neuroscience] Re: Differential Attenutation of EPSPs in Voltage
Clamp vs Current Clamp
(by r_s_norman from comcast.net)
Fri Aug 7 08:33:45 EST 2009
On Fri, 7 Aug 2009 02:15:31 -0700 (PDT), Bill
<connelly.bill from gmail.com> wrote:
>I was reading a paper by Stephen Williams that showed that you get
>more distance dependent attenuation of EPSPs when you record in
>voltage clamp than in current clamp. I modelled it using NEURON and I
>got the exact same result. Why is this?
>I would have thought the (albiet partial) voltage control along the
>cable would mean that voltage clamp would reduce capacitive currents
>(i.e. less C*dv/dt), and therefore would retain more longitudial
>current and you would get less attenuation.
It is easier to see this doing the rather complex math, but here is an
attempt at an explanation.
With current clamp, there is essentially no "loading" or alteration of
the conditions for voltage and current spread down a cable and the
voltage decays with distance in a particular fashion.
With voltage clamp, there is essentially a "short circuit" across the
cable at the point of the clamp. This causes a rather drastic
alteration in the pattern of current flow pattern when the clamp is
close to the synapse. However when the clamp is farther down the
cable, the current flow near the synapse is virtually unchanged. As a
result, when the clamp is near the synapse it "steals" pretty much all
the current, far more than would pass that way unclamped. When the
clamp is far from the synapse, it only gets what current would
normally end up passing that far anyway. Hence an apparent "faster
decay with distance" in voltage clamp than unclamped.
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