[Neuroscience] Re: Warm slicing

Bill.Connelly via neur-sci%40net.bio.net (by connelly.bill from gmail.com)
Sun Aug 16 21:08:14 EST 2009


For me it was death city when I tried it on 30 day old animals.


On Aug 17, 12:31 am, Jose Guzman <n... from neurohost.org> wrote:
> Bill wrote:
> > Hi Guys,
>
> > I thought I would post the result of a little experiment I tried
> > today. After reading:
> >http://www.precisionary.com/products_faq.html#q9
> > I tried making some cortical slices (250um thick) in a cutting chamber
> > heated with a 35 degree water bath (in sucrose aCSF) from 20 day old
> > tissue. While the brain was so much more fluid than at 4 degrees, but
> > the cells were wonderfully visible and healthy looking.
> > Just a suggestion that I thought someone might be interested in.
>
> Hi Bill,
>
> I though already about cutting with a warm bath... but I did not want to
> risk... on the other hand P20 are quite easy to get, what about older
> animals? (ie. 1/2 months?)



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