[Neuroscience] Chromatin IP protocol from mouse brain

Sébastien Vigneau via neur-sci%40net.bio.net (by sebastien.vigneau from gmail.com)
Mon Dec 28 13:48:01 EST 2009


Hi,

Does anyone have a good protocol for cross-linked chromatin
immuno-precipitation from 3-5 weeks mice (including the chromatin
preparation part)? I have tried two of them, but find it difficult to purify
chromatin with a good yield, and at the same time get it clean enough to
allow for reproducible immuno-precipitation results.

Also, does anyone know what is a reasonable quantity of chromatin to expect
from a single brain?

Thank you for your help,

Sébastien Vigneau


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